Skip to main content
. 2015 Oct 23;6:8669. doi: 10.1038/ncomms9669

Figure 5. ABI5 accumulation and degradation in prt6-1 mutant background during seed germination and post germination.

Figure 5

(a,b) ABA treatment promotes ABI5 accumulation in germinating seeds. Immunoblot analysis of ABI5 protein levels in seed extracts of Col-0 and prt6-1 before (a) and after 48 h treatment with (+) or without (−) 0.25 μM ABA (b). Actin protein levels are shown as a loading control. (c) Post-germinative ABI5 accumulation in seedlings. Immunoblot analysis of ABI5 protein levels in 10-day-old extracts of Col-0, prt6-1 and abi5-1, treated with or without (−) 0.25, 0.5 and 1 μM ABA. Actin protein levels are shown as a loading control and Rubisco large subunit (RbcL) detection is indicated. (d) ABI5 protein levels in wild-type (Col-0), prt6-1 and abi5-1 mutant backgrounds. Stratified seeds were incubated with 5 μM ABA for 48 h (T0) and treated after ABA removal with H2O, ABA, SNAP (1 mM) and GSNO (1 mM) for 12 h. Immunoblot analysis of ABI5 protein levels in seed extracts of wild type and mutants. Actin protein levels are shown as a loading control.