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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Mol Microbiol. 2015 Sep 10;98(4):681–693. doi: 10.1111/mmi.13150

Fig. 2. tRNA-dependent lipid alanylation by Cg1103.

Fig. 2

tRNA-dependent lipid alanylation activity was measured in vitro using either the purified construct Cg1103483–832 (lanes 1–3), or a membrane preparation from C. glutamicum harboring pEKEx2-Cg1103 (lanes 6 and 7). The lipids used as aa acceptors were a total lipid preparation isolated from C. glutamicum (CG), or commercial egg-PG (PG). Lipid products were analyzed by TLC using solvent A for the mobile phase. Activity with a membrane extract isolated from E. coli cells expressing RakPGS from E. faecium is shown as a positive control for [14C]Ala-PG synthesis (lane 5). O: TLC origin, F: solvent front, Ala-UL: alanylated unknown lipid.