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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Mol Microbiol. 2015 Sep 10;98(4):681–693. doi: 10.1111/mmi.13150

Fig. 4. ESI/MS/MS analysis of amine-containing lipids in C. glutamicum.

Fig. 4

(A) Lipids from wild-type C. glutamicum (wt), and a strain harboring the plasmid pEKEx2-Cg1103, were analyzed by TLC using solvent A as the mobile phase. Total lipids were revealed using iodine vapor (I2), and amine and phosphorus containing lipids were revealed using ninhydrin (N) and Dittmer reagent (DR), respectively. Amine-containing lipids (ACL1 and ACL2) were purified using a two-step flash chromatography procedure as described in methods (see Fig. S4 for fractionation data) and analyzed by mass spectrometry. ESI/MS/MS spectra of fractions containing ACL1 (B) and ACL2 (C) revealed molecular ion and fragmentation peaks consistent with m/z values for Ala-DAG (665.56) and Ala-PG (810.56), respectively. CL: cardiolipin, PG: phosphatidylglycerol, O: TLC origin, F: solvent front.