Skip to main content
. 2015 Oct 12;125(11):4239–4254. doi: 10.1172/JCI81203

Figure 1. Reduced STK4 expression is associated with DEN-induced HCC and excessive inflammation.

Figure 1

(A–C) WT male mice were treated with controls (Veh) or DEN (50 mg/kg) to induce HCC and were sacrificed 11 months after DEN injection (n = 3). (A) HE staining (scale bar: 100 μm) or IHC staining with anti-F4/80 (scale bar: 50 μm) of mouse livers. (B and C) Percentages of intrahepatic MoMs or KCs, and STK4 expression levels were analyzed by FACS. (D) Eleven months after Veh (n > 4) or DEN (n > 7) treatment, serum IL-6 concentrations were detected by ELISA. The correlation of Il6 and Stk4 mRNA levels in intrahepatic immune cells were analyzed by Spearman’s test (n = 9). (E and F) Ten hours after LPS challenge (3 mg/mouse, i.p.) of WT (n = 3) and Stk4–/– mice (n = 3), HE staining of lungs (scale bar: 100 μm) or serum IL-6 and TNF-α concentrations were measured. Values are mean ± SD from 2 independent experiments. Data represent mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001, using 2-tailed, unpaired Student’s t test (B–D) or 1-way ANOVA with Holm-Sidak’s multiple comparisons test (F).