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. 2015 Sep 28;125(11):4063–4076. doi: 10.1172/JCI82152

Figure 4. LKB1 loss in endometrium promotes recruitment of macrophages that express markers associated with alternative macrophage activation.

Figure 4

Experiments were conducted in 12-week-old animals at proestrus. (A) Macrophage density by F4/80 immunohistochemistry. (B) Quantitation of macrophages in uterine tissue sections immunostained for F4/80. Positive cells were counted in 5 separate fields and normalized by total area for every mouse analyzed (n = 5 for Lkb1+/+, n = 6 for Lkb1–/– mice). There were significantly increased macrophage numbers in Lkb1–/– endometrium. *P < 0.005, per Student’s t test. (C) Presence of alternatively activated macrophages characterized by ARG1 and CD163 expression. Uterine tumor sections were stained with F4/80 and CD163 or ARG1. Arrows in the inset highlight F4/80 cells that are also positive for the other markers. Scale bars: 50 μm. Error bars = SEM.