Figure 6. TIGIT signaling in Tregs.

CD4⁺ FOXP3⁺ Tregs from WT Foxp3-GFP–KI mice were sorted and stimulated with plate-bound isotype or agonistic anti-TIGIT Ab, together with anti-CD3 and anti-CD28, for 48 or 96 hours. Gene expression was then analyzed using a NanoString CodeSet. Bar graphs show the fold expression ± SEM of genes in Ab-treated versus isotype-treated cells at 48 and 96 hours. Data were pooled from 2 different experiments.