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. 2015 Oct 5;125(11):4122–4134. doi: 10.1172/JCI82423

Figure 7. FOXO1 mediates the function of CIP in cardiomyocyte hypertrophy and cardiac remodeling.

Figure 7

(A) Neonatal rat cardiomyocytes were transfected with siRNA for CIP or control siRNA. Cells were treated or not treated with PE, and the expression of CIP and FOXO1 proteins was examined by Western blotting. GAPDH was used as a control. (B) Immunofluorescence images of α-actinin staining in neonatal rat cardiomyocytes with (AdFOXO1) or without (AdGFP) FOXO1 overexpression. Scale bar: 30 μm. (C) Quantification of cardiomyocyte size. More than 100 cardiomyocytes were measured for each experimental group. (D) qRT-PCR detection of the expression of Anp, Bnp, and Acta1 in cardiomyocytes. Data were obtained from 3 independent experiments. *P < 0.05, **P < 0.01, 1-way ANOVA with post-hoc Tukey’s test. (E) A working model postulating the function of CIP in cardiac remodeling and disease.