At 90 minutes after infection, in cells under the indicated treatment conditions (IFN-β, 1,000 units/ml; tubacin, 5 μM), colocalization of GFP fluorescence either (A) with RFP fluorescence (RFP-LAMP1) or (B) with DAPI fluorescence (perinucleus) was counted in each cell after they were fixed with 4% paraformaldehyde and stained with DAPI. The horizontal bars and error bars represent average and mean ± SD, respectively (n = 22–27; *P < 0.05, **P < 0.01, ***P < 0.001 by 1-way ANOVA test). Numbers of cells used for counting GFP dots are as follows: –tubacin/–IFN-β, n = 27; +tubacin/–IFN-β, n = 26; –tubacin/+IFN-β, n = 22; and +tubacin/+IFN-β; n = 23.