Skip to main content
. 2015 Oct 20;125(11):4269–4280. doi: 10.1172/JCI80713

Figure 5. Time-lapse imaging after infection of U251 glioma cells with a VP26-GFP–expressing HSV-1 in tubacin-treated and/or IFN-β–treated RFP-LAMP1–expressing U251 cells.

Figure 5

VP26-GFP–expressing HSV-1 (K26GFP) was added to RFP-LAMP1 stably expressing U251 cells after (C, D, G, and H) IFN-β pretreatment and/or (B, D, F, and H) tubacin treatment. GFP/RFP fluorescence signals were captured using time-lapse confocal microscopy. (A–D) The time at which each image was taken after infection is indicated on the top left and the bottom right. Scale bar: 20 μm. (E–H) Blue bars represent the percentages of intranuclear GFP puncta in total GFP-positive cells. Gray bars indicate that the presence of GFP-positive cells is 100%. Total counts of GFP-expressing cells and time (h.p.i.) after the infection are shown at bottom of bar plots. (See also Supplemental Figure 2.)