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. 2015 Oct 5;125(11):4107–4121. doi: 10.1172/JCI78182

Figure 5. pDCs exposed in vitro to a PR3-induced microenvironment triggered a skewed Th2/Th9 cell distribution.

Figure 5

(A) Representative dot plots demonstrating FOXP3 expression in CD4+CD25+ T cells and IFN-γ and IL-17A expression in CD4+ T cells. Flow cytometry analysis of naive CD4+CD25– OVA-specific OTII T cells cultured for 4 days with pDCs preexposed to macrophage supernatants generated from medium alone (white triangles), apoptotic controls (white circles), or apoptotic PR3-expressing cells (black squares). (B) Percentages of Tregs (CD4+CD25+FOXP3+), Th1 cells (CD4+IFN-γ+), and Th17 cells (CD4+IL-17A+) obtained following pDCs cocultured with naive T cells. (C) IL-4, IL-5, IL-9, IL-17, and IFN-γ secretion measured in media collected from the coculture experiments using a Luminex assay. Values are shown as mean ± SEM; n = 5 mice per group, each parameter determined in triplicates. *P < 0.05; **P < 0.01; ***P < 0.001. Significant differences between groups were determined by multicomparison ANOVA (B and C).