Figure 8. Neutrophils, pDCs, and macrophages were located in granulomatous inflammation from GPA patients.

(A) Double-positive CD123 (red staining) and IFN-α cells (brown staining) were located within the granulomatous lesion of lung tissue from GPA patients, and nuclei were counterstained in blue (black arrows). (B) IFN-α⁺ (brown staining) and CD123⁺ cells (red staining) were detected at the edge of a neutrophilic abscess in GPA (black arrow). (C–E) Serial sections of a neutrophilic microabscess showed PR3⁺ (C, brown staining) and LL37⁺ cells (C, red staining) surrounded by CD68⁺ macrophages (D, red staining) and IFN-α⁺ cells (E, brown staining). Lower panels depict higher magnification of the area inside the box in the upper panels. Data display typical images representative of 6 GPA patients. Scale bars: 50 µm (A, C–E, upper panels); 20 µm (B, C–E, lower panels).