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. 2015 Oct 30;6:8719. doi: 10.1038/ncomms9719

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(a,b) BMP signalling was detected with an antibody against phosphorylated Smad1/5/8 (green) (a) in an embryo injected with a control LacZ MO, and (b) in an embryo injected with an Admp MO. (c,d) DAPI staining shows nuclei (blue) of the embryos shown in a and b. (e,f) Expression of Bmp2/4 at the late-gastrula stage is observed in the ventral ectoderm in an embryo injected with a control LacZ MO (e), while it is lost in an embryo injected with an Admp MO (f). Scale bar, 100 μm (a). The number of embryos examined and the proportion of embryos that each panel represents are shown within the panels. DAPI, 4,6-diamidino-2-phenylindole.