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. 2015 Oct 12;8(10):e22413. doi: 10.5812/jjm.22413

Figure 3. Agarose Gel Electrophoresis of Polymerase Chain Reaction Products of tet (K and M) and erm (A and C) Genes.

Figure 3.

Lanes M: 100 bp DNA marker (Fermentas, Germany); Lane 1: positive control for tetK gene of amplified 360-bp DNA (S. aureus ATCC BAA-39); Lane 2: negative control (reaction mixture without DNA); Lanes 3 - 5: S. aureus isolates positive for tetK gene; Lane 6: positive control for tetM gene of amplified 158-bp DNA fragment (S. aureus T0131); Lane 7: negative control (reaction mixture without DNA); Lanes 8 - 10: representative S. aureus nasal isolates negative for tetM; Lane 11: the only S. aureus isolate positive for tetM gene; Lane 12: positive control for ermA gene of amplified 190-bp DNA (S. aureus T0131); Lane 13: negative control for ermA gene; Lanes 14 - 16: representative S. aureus nasal isolates negative for ermA gene; Lane 17: positive control for ermC gene of amplified 299-bp DNA (S. aureus 71193); Lane 18: negative control for ermC gene; Lanes 19 - 21: representative S. aureus nasal isolates negative for ermC gene.