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. 2015 Oct 28;6:8759. doi: 10.1038/ncomms9759

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(a) RNA in situ hybridization for Fur1 and Fur2 in stage 10 egg chambers reveals both genes are expressed in the nurse cells (nc) and oocyte (oo). Sense controls show no staining. (b) Whole larval cuticles and head skeletons of animals with maternal knockdown (nos-Gal4) of Fur1 and Fur2 transcripts using UAS-shRNAs. Knockdown of either Fur1 or Fur2 alone has no effect. Co-knockdown of Fur1 and Fur2 results in terminal defects including a reduced posterior filzkorper (fz, arrowed) and head skeleton defects (arrowheads) similar to embryos laid by trk^Δ females. Anterior is to the left. Scale bars, 50 μm. Images are representative of at least two repeat experiments, with at least 100 individuals observed per genotype.