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. 2015 Nov 4;8:3245–3253. doi: 10.2147/OTT.S90012

Figure 2.

Figure 2

miR-21 reversed the change of apoptosis, migratory and invasive abilities induced by NS398 in AGS cells.

Notes: AGS cells were transfected with miR-21 mimics and treated with NS398 (100 μM/L). (A) The percentage of apoptotic cells was determined by flow cytometry. miR-21 mimics decreased the apoptosis induced by NS398. (B) The percentage of apoptotic cells was determined by TUNEL assay. miR-21 mimics decreased the apoptosis induced by NS398. (C) The migratory ability of AGS cells was evaluated by wound-healing assay. The wound widths of each sample were measured under phase-contrast microscopy (Olympus, Tokyo, Japan). (D) The invasion activities were detected by transwell assay. Representative image fields of invaded cells on the membrane are shown. (E) miR-21 expression was detected by qRT-PCR, after miR-21 mimics were transfected into AGS cells for 24 hours. miR-21 mimics significantly increased miR-21 expression in gastric cancer (GC) cells. Magnification in figures C–E is ×200. The images shown are representative of three experiments. Values are means ± SEM, n=6. *P<0.05.

Abbreviations: TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling; qRT-PCR, quantitative real-time polymerase chain reaction; SEM, standard error of the mean; miR, microRNA; NC, negative control; FITC, fluorescein isothiocyanate.