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miR-21 mediates the anticancer effects of NS398 by regulating the protein expression ratio of Bcl-2/Bax and PTEN.
Notes: After AGS cells were transfected with miR-21 mimics and treated with NS398 (100 μM/L) the expression levels of Bcl-2, Bak, Bax, cleaved caspase-3, and PTEN were measured by Western blot. Quantitative analysis was performed with beta-actin as the internal control (*P<0.05).
Abbreviations: miR, microRNA; NC, negative control.
