Fig. 5.

IRAK4 mediated the suppressed inflammatory response induced by miR-367 in erythrocyte lysate-treated microglia. a Detection of the inhibition efficiency of siRNAs against IRAK4. Microglia was transfected with scramble siRNA or IRAK4 siRNA and then exposed to erythrocyte lysates. After 48 h, IRAK4 protein expression was analyzed using western blots. b Microglia was transfected with scramble siRNA or IRAK4 siRNA and then exposed to PBS or erythrocyte lysates. After 48 h, IRAK4 and NF-κB p65 protein expression were analyzed using western blots. c Microglia was transfected with scramble siRNA or IRAK4 siRNA and then exposed to PBS or erythrocyte lysates. After 48 h, IL-6, IL-1β, and TNF-α mRNA and protein expression were evaluated by RT-PCR and ELISA. Experiments performed in triplicate showed consistent results. Data are presented as the mean ± SD of three independent experiments. *P < 0.05