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. Author manuscript; available in PMC: 2016 May 5.
Published in final edited form as: Oncogene. 2015 Mar 2;34(45):5617–5625. doi: 10.1038/onc.2015.32

Figure 1. Erastin induces HSF1-dependent HSPB1 expression in human cancer cells.

Figure 1

(A) HeLa cells were treated with erastin (0.5 µM) for eight hours and 24 hours, and mRNA expressions of indicated HSPs were assayed by Q-PCR (n=3). (B) Indicated human cancer cells were treated with erastin (HeLa, 0.5 µM; U2OS, 5 µM; LNCaP, 5 µM) for 24 hours and the protein expressions of indicated HSPs were assayed by Western blot. (C, D) Indicated human cancer cells were transfected with control shRNA and HSF1 shRNA for 48 hours and treated with erastin (HeLa, 0.5 µM; U2OS, 5 µM; LNCaP, 5 µM) for 3–24 h. The mRNA expressions of HSPB1 (C) were assayed by Q-PCR (n=3, *p < 0.05 versus control shRNA group). In parallel, the protein expressions of HSPB1 and HSF1 (D) were assayed using Western blot.