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. Author manuscript; available in PMC: 2015 Nov 10.
Published in final edited form as: Adv Healthc Mater. 2015 May 4;4(10):1484–1423. doi: 10.1002/adhm.201400841

Figure 1.

Figure 1

Synthetic polymer nanoparticles are loaded with nitric oxide (NO) through the formation of physiological NO adducts, S-nitrosothiols (SNO), with NP thiols (SH). (a) NP synthesis and S-nitrosation scheme to create S-nitrosothiol (SNO) NP (SNO-NP). (b) Particle SH concentration is controlled by initiator concentration during emulsion polymerization synthesis. (c) NP diameter remains unchanged by SH concentration and NO loading. (d) SNO concentration and number per NP is proportional to NP SH concentration. NP-encapsulated NO is in the form of SNO as demonstrated by UV absorbance peaks at 340 (e) and is NP bound (f). (e) Arrow indicates increasing SNO-NP concentration. Data shown for a highest SNO-NP concentration of 5.6 mM with approximate 2 fold dilutions. Inset demonstrates calculation of molar extinction coefficient that was found to be 540 M-1cm-1. (g) Whereas nitrite (NO2-) remaining after S-nitrosation of SH-NP is rapidly dialyzed away, SNO signal by the Saville assay is retained.