(A) Generation of a modified ERβ bacterial artificial clone that has a Gal4 DNA-binding domain and the Gal4-UAS-hrGFP reporter. DY380, Bacterial recombination strain. KanR, kanamycin-resistant gene. DBD, DNA-binding domain. Gal4-UAS, Gal4-upstream activating sequence. FLP, flippase. hrGFP, humanized renilla GFP.
(B) IHC analyses of hrGFP levels in the uteri of sham-treated ERBAI mice and ectopic and eutopic endometria of ERBAI mice with endometriosis.
(C and D) The quantification of hrGFP levels in the epithelial (C) and stromal compartment (D) of each type of endometrium in panel B. See also Figures S1.