Fig. 3.

Prior treatment with anticancer agents significantly diminishes chemotaxis toward CXCL12. Cells were pre-treated either with vehicle control or 2 μg/mL a 5-FU; b Cis; c Vin; or d MTX for 48 h, isolated from culture and then assayed for chemotaxis toward 100 ng/mL CXCL12 (hatched bars) or vehicle control (light bars). Mean ± SE (n = 6). *, P < 0.01 by ANOVA. e CXCL12-dependent loss of CXCR4 cell-surface expression. f cell-surface CD26 is unaffected by CXCL12