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. 2015 Nov 9;16:341. doi: 10.1186/s12891-015-0799-4

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© Mern and Thomé. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Fluorescence micrographs of the initial scAAV serotype screening on day 8 of post-transduction. For the primary screening of the serotypes in NP cells, 1 x 10⁵ NP cells were seeded and transduced with GFP packing scAAV serotypes at different viral genome copies per seeded cell (vg/c) and fluorescence micrographs were acquired up to 48 days. On day 8 of post-transduction scAAV2, scAAV3 and scAAV6 displayed the highest transduction efficiencies at a dose of 5000 vg/c. Original magnification: x 40