Table 4.
Levels of inflammatory, catabolic and matrix proteins in scAAV6 treated NP cells of DDG III
| Target protein | NP cell sample | Mean [pg/ml] | SD | Mean fold |
|---|---|---|---|---|
| IL-1β | control | 110 | 2.247 | 1.009 |
| scAAV6 | 109 | 1.702 | ||
| TNF-α | control | 93 | 1.648 | 1.021 |
| scAAV6 | 91 | 2.314 | ||
| ADAMTS-4 | control | 1807 | 79.49 | 1.029 |
| scAAV6 | 1756 | 105.6 | ||
| ADAMTS-5 | control | 4156 | 177.9 | 1.028 |
| scAAV6 | 4042 | 203.5 | ||
| Aggrecan | control | 29100 | 1112 | 1.010 |
| scAAV6 | 28804 | 1279 | ||
| Collagen II | control | 10311 | 542.7 | 1.013 |
| scAAV6 | 10171 | 548.9 |
1 x 105 NP cells were monolayer transduced with scAAV6 [5000 vg/c] and 3D cultured for four weeks in collagen I scaffold. Expression levels [pg/ml] of the inflammatory cytokines (IL-1β & TNF-α), catabolic factors (ADAMTS-4 & ADAMTS-5) and matrix proteins (Aggrecan & Collagen II) were determined using ELISA-assays applied on 100 μg of total protein extracts of each sample. Data represent the mean and standard deviation (SD) of three independent experiments, each performed in duplicate (p < 0.05). The mean fold represents the ratio of the untreated (control) expression level to scAAV6 treated expression level