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. 2015 Nov 9;6:219. doi: 10.1186/s13287-015-0207-x

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© Qu et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Subretinal transplantation of EGFP labeled rESC-RPCs in the RCS model. a Generation of rESC-RPC2 from EF1α::EGFP lentivirus labeled rESCs. Scale bars: 50 μm. b Immunofluorescence staining of EGFP-rESC-RPC2 at D16 before transplantation. Antibody against EGFP (green) and antibodies against Nestin/Nes and Tuj1 (red) were used. DAPI (blue) was used to highlight the nuclei. Scale bars: 50 μm. c Flow cytometric analysis of EGFP-rESC-RPC2 in comparison with unlabeled rESC-RPC2. d Grafted rESC-RPC2 could be detected in the retina at different times after transplantation. GCL ganglion cell layer, INL inner nuclear layer, ONL outer nuclear layer. Scale bar: 50 μm. EGFP enhanced green fluorescent protein, rESC rat embryonic stem cell, RPC retinal progenitor cell, RCS Royal College of Surgeons, DAPI 4′,6-diamidino-2-phenylindole