Figure 3.

Glutamic acid excitotoxicity model; poly-arginine peptide treatment before or during glutamic acid exposure. (A) Peptides present in neuronal cultures for 10 minutes only before glutamic acid exposure. (B) Peptides present in neuronal cultures only during the 5-minute glutamic acid exposure. (C) Peptides present in neuronal cultures for 10 minutes only at 1 to 5 hours before glutamic acid exposure. Neuronal viability measured 20 to 24 hours after glutamic acid exposure. Concentration of peptide in μmol/L. MTS data were expressed as percentage neuronal viability with no insult control taken as 100% viability (mean±s.d.; n=4; *P<0.05). MTS, 3-(4,5,dimethyliazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfophenyl)-2H-tetrazolium salt.