. 2015 May 28;4(Suppl 1):51–58. doi: 10.1159/000430840

Table 2.

Interday validation outcome for TH and TAM extraction

Interday (n = 10)/hormone	Linear regression		HQAL (80 nM)			MQAL (10 nM)			LQAL (0.75 nM)			LLOQ, pM	LLOD, pM
	related IS	R² range (n = 4)	mean conc., nM %	CV, %	accuracy, %	mean conc., nM	CV, %	accuracy, %	mean conc., nM	CV, %	accuracy, %
T₀	¹³C₆-3,3′-T₂	0.996–0.999	82.97	7.9	103.7	11.41	9.8	114.1	2.29	33.0	296.2	–	–
3-T₁	¹³C₆-3,3′-T₂	0.991 – 0.997	80.17	5.0	100.2	10.20	5.5	102.0	0.79	13.7	102.8	78	30
3′-T₁	¹³C₆-3,3 ′-T₂	0.994 – 0.998	80.47	5.3	100.6	10.36	4.8	103.6	0.78	8.7	101.1	39	15
3,5-T₂	¹³C₆-3,3 ′-T₂	0.997 – 0.999	77.36	4.9	96.7	10.33	5.4	103.3	0.77	8.5	100.4	78	30
3,3′ -T₂	¹³C₆-3,3 ′-T₂	0.997 – 0.998	79.76	5.7	99.7	10.34	6.6	103.4	0.77	8.9	100.5	78	15
3 ′, 5 ′-T₂	¹³C₆-3,3 ′-T₂	0.994 – 0.998	80.70	6.7	100.9	10.43	7.7	104.3	0.77	9.5	100.5	78	30
T3	¹³C₆-T3	0.998 – 0.999	78.91	8.1	98.6	10.24	7.6	102.4	0.77	7.7	100.1	78	15
rT₃	¹³C₆-rT3	0.994 – 0.999	83.08	5.2	103.9	10.36	6.6	103.6	0.79	8.6	103.0	78	15
T₄	¹³C₆-T4	0.995 – 0.998	75.28	12.2	94.1	9.99	11.0	99.9	0.75	10.8	97.7	78	15

T₄AM	²H4-3-T1AM	0.994 – 0.997	90.56	12.0	113.2	11.08	11.2	110.8	0.82	10.9	106.7	234	78
t₃am	²H4-3-T1AM	0.998 – 0.999	85.68	7.2	107.1	10.90	8.9	109.0	0.82	10.1	106.0	39	15
rT3AM	²H4-3-T1AM	0.998 – 0.998	88.25	6.4	110.3	11.41	7.2	114.1	0.85	9.6	110.3	78	15
3,5-T2AM	²H4-3-T1AM	0.997 – 0.998	85.23	5.1	106.5	10.98	7.3	109.8	0.82	7.9	106.9	78	15
3-T1AM	²H4-3-T1AM	0.997 – 0.999	84.28	4.6	105.4	10.92	7.1	109.2	0.82	8.5	105.8	78	15
T₀AM	²H4-3-T1AM	0.994 – 0.998	85.11	4.8	106.4	10.96	6.6	109.6	0.87	10.1	112.3	78	59

R² = Coefficient of determination; mean conc. = mean determined concentration. DMEM/F12 contains approximately 1.54 nM T₀ as endogenous background concentration.