Table 3.

Intraday validation outcome for TH and TAM extraction

Intraday (n = 10)/ analytes	HQAL (80 nM)			MQAL (10 nM)			LQAL (0.75 nM)
	mean conc., nM	CV, %	accuracy, %	mean conc., nM	CV, %	accuracy, %	mean conc., nM	CV, %	accuracy, %
T0	82.61	3.7	103.3	12.62	2.1	126.2	2.93	1.9	391.1
3-T1	77.08	2.7	96.4	10.62	1.9	106.2	0.82	4.1	108.7
3′-T1	73.07	2.6	91.3	10.23	2.9	102.3	0.82	3.9	109.7
3,5-T2	71.71	1.6	89.6	10.16	3.1	101.6	0.80	2.7	106.6
3,3′-T2	73.67	2.7	92.1	9.94	2.9	99.4	0.79	3.6	104.7
3′,5′-T2	75.24	2.3	94.0	10.51	3.3	105.1	0.81	3.9	107.8
T3	71.21	2.4	89.0	9.70	3.0	97.0	0.76	3.1	100.7
rT3	78.45	2.3	98.1	10.36	2.6	103.6	0.77	3.1	102.4
T4	72.20	2.0	90.2	9.62	4.1	96.2	0.72	6.4	96.5
t4am	90.07	4.6	112.6	11.60	2.5	116.0	0.89	5.1	119.2
t3am	84.32	1.8	105.4	11.34	2.5	113.4	0.88	3.5	116.7
rT3AM	83.66	2.1	104.6	11.35	2.9	113.5	0.86	4.5	114.0
3,5-T2AM	83.55	2.0	104.4	11.25	2.2	112.5	0.86	3.5	115.2
3-T1AM	82.72	2.6	103.4	11.31	2.8	113.1	0.88	3.1	117.3
T0AM	88.76	1.2	111.0	11.50	1.5	115.0	1.02	3.9	111.2

R² = Coefficient of determination; mean conc. = mean determined concentration. DMEM/F12 contains approximately 1.54 nM T₀ as endogenous background concentration.