Fig. 2.

Effect of aging on TH transporter mRNA expression in liver tissues of old compared to young euthyroid male mice. Gene expression levels were determined by quantitative real-time PCR in livers of old euthyroid mice (21.8 months) and were normalized to respective expression levels in livers of euthyroid young mice (5.8 months). Mct10, Lat1, Lat2, Oatp1b2, Oatp1a4 and Ntcp mRNA expression levels are significantly upregulated in liver tissues of old mice compared to young animals. 18S, Ppia and Rpl13a were used as reference genes. Data are presented as fold changes, means ± SEM; n = 7; efficiency-corrected ΔΔCt method; t test, * p < 0.05, ** p < 0.01.