Figure 4.
Effect of shear on the expression and posttranslational modification of interendothelial junction proteins in human brain microvascular endothelial cells (HBMvECs). Confluent cells were exposed to steady laminar shear stress (8 dynes/cm2, 24 hours). After treatment, total cell protein and mRNA was harvested for immunoprecipitation (IP)/Western blotting and RT-qPCR analysis, respectively. Upper histograms represent (A) the fold change in mRNA and (B) the densitometric fold change in protein levels for occludin, claudin-5, and VE-cadherin in response to shear (bars reading left to right). *P⩽0.05 versus static control. Lower histograms show the densitometric fold change in relative (C) pTyr and (D) pThr levels for occludin, claudin-5, and VE-cadherin in response to shear (bars reading left to right). *P⩽0.05 versus static control. All blots are representative. Cl-5, claudin-5; Occ, occludin; VE-Cad, VE-cadherin.