Figure 6.

Effect of shear preconditioning on cytokine-induced ROS generation in human brain microvascular endothelial cells (HBMvECs). Confluent cells were preconditioned for 24 hours under static or shear conditions (0 or 8 dynes/cm², 24 hours) before treatment of cells with tumor necrosis factor-α (TNF-α, A) or interleukin-6 (IL-6; 100 ng/mL, 6 or 18 hours; B). Static and shear conditions were maintained during cytokine treatments. ROS production was subsequently monitored by flow cytometry using 2′,7′-dichlorofluorescein diacetate (CFDA). Histograms (LHS) represent the fold change in fluorescent signal normalized to untreated control at 6 or 18 hours. Representative flow cytometry scans (RHS) are also shown for both 6- and 18-hour cytokine treatments in the absence and presence of shear. Gray-shaded scan indicates untreated control (full key beneath scans). *P⩽0.05 versus untreated 6 or 18 hour controls. ^ØP⩽0.05 versus cytokine without shear. LHS, left hand side; RHS, right hand side.