Figure 7.
Effect of shear on NADPH oxidase activation in human brain microvascular endothelial cells (HBMvECs). Confluent HBMvECs were exposed to steady laminar shear stress (8 dynes/cm2, 24 hours). After treatment, cells were harvested for analysis of NADPH oxidase subunit expression and coassociation, as monitored by Western blotting and co-IP, respectively. (A) Histograms represent densitometric fold change in protein levels for gp91phox and p47phox in response to shear. *P⩽0.05 versus static control. (B) Histograms represent the densitometric fold change in gp91phox/p47phox coassociation in response to shear. LHS histogram=IP p47phox, WB gp91phox; RHS histogram=IP gp91phox, western blot p47phox. *P⩽0.05 versus static control. All blots are representative. IP, immunoprecipitation; LHS, left hand side; RHS, right hand side.