Table 3.

qRT-PCR test results of 19 genes detected by RNA-sequencing

Gene description	Uniport_ID	Floral Bud	Flower
Actin depolymerizing factor 10	B9I489_POPTR	1.35E-04***	2.44E–03**
Arabinogalactan peptide 20	AGP20_ARATH	8.00E–04***	2.10E–04***
Blue copper protein (Fragment)	O82576_MAIZE	1.87E–04***	1.56E–03**
Calcium–dependent protein kinase 2	Q3YAT0_PETIN	1.10E–04***	4.03E–04***
Callose synthase 5	C8C9X3_ARATH	8.66E–05***	2.26E–02*
CBL–interacting protein kinase 16	C4P7W5_VITVI	2.92E–04***	1.92E–04***
Cyclin–like protein	Q6ZIX9_ORYSJ	2.59E–05***	6.36E–04***
Elongation factor 1–alpha	B9SPV9_RICCO	2.75E–03**	7.44E–04***
Late embryogenesis abundant protein 1	LEA1_CICAR	1.41E–04***	2.35E–04***
LIM domain protein	Q306K1_BRANA	8.75E–04***	5.13E–06***
MADS–box transcription factor 1	D1MFS6_HEVBR	1.99E–03**	6.63E–05***
Mitotic spindle assembly checkpoint protein MAD2	D2V3A0_NAEGR	5.34E–06***	8.07E–03**
Nucleotide sugar epimerase	B1WNM2_CYAA5	1.02E–02*	8.12E–04***
Pectate lyase	B6TSP4_MAIZE	7.71E–03**	2.84E–04***
Polygalacturonase	E3VSV7_CUCPE	1.42E–03**	1.96E–04***
Rho GDP–dissociation inhibitor family protein	D7L724_ARALL	4.51E–03**	9.35E–04***
S–adenosylmethionine synthase	B9S0G1_RICCO	2.14E–03**	6.32E–04***
Subtilisin–like protease	Q6WNU4_SOYBN	1.86E–02*	1.93E–02*
Zinc finger family protein	D7MD22_ARALL	3.19E–03**	5.41E–04***

Student’s t–test was used for separating the differences between a male sterile line (DAH3615–MS) and a male fertile line (DAH3615) in floral buds and flowers. *P < 0.05, **P < 0.01, ***P < 0.001