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. Author manuscript; available in PMC: 2015 Nov 10.
Published in final edited form as: Methods Mol Biol. 2015;1334:313–332. doi: 10.1007/978-1-4939-2877-4_20

Figure 1.

Figure 1

Representative SPR sensorgrams with three major stages: (1) initial buffer flow for a stable reference baseline; (2) ligand association phase with sample injections over the sensor chip surface; (3) ligand dissociation phase with buffer flow over the surface.