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. Author manuscript; available in PMC: 2015 Nov 10.

Published in final edited form as: Methods Mol Biol. 2015;1334:313–332. doi: 10.1007/978-1-4939-2877-4_20

Table 3.

Binding affinities and kinetics for PU.1 with and without non-specific (NS) DNA at 400 mM NaCl^*.

	K_a (× 10⁶ M⁻¹ S⁻¹)	k_d (S⁻¹)	K_D (nM)
	K_a (× 10⁶ M⁻¹ S⁻¹)	k_d (S⁻¹)	Kinetic fit	Steady-state fit
No NS DNA	31 ± 1	0.22 ± 0.01	7.0 ± 0.2	6.8 ± 0.3
300 µM NS DNA	2.4± 0.1	0.18± 0.01	73± 1	63 ± 2

^*

Errors listed in this table are standard errors for the fit of 1:1 binding model.