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. 2015 Nov 9;8:577. doi: 10.1186/s13071-015-1197-3

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© Paiva et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Collagen measurement and analysis of GR-MF spheroids a Collagen measurement into supernatants from non-stimulated or TGF-β (30 ng/mL) stimulated cells after 24 h of culture in monolayer b Representative photomicrographs of spheroids stained with haematoxylin/eosin (HE) and Modified Sirius Redstaining for collagen, optical microscope analyses (middle column) and confocal microscope analyses (right column). c Collagen measurement into supernatants from nonstimulated, TGF-β (30 ng/mL) or IL-13 (40 ng/mL) stimulated spheroids after 4 days of culture. Each bar represents the mean ± SEM, n = 3. This graph is representative from three experiments. *P ≤ 0.05 compared with non-stimulated cells