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. 2015 Nov 9;8:577. doi: 10.1186/s13071-015-1197-3

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© Paiva et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Eotaxin expression by GR-MF a Real time RT-PCR to determine the relative expression of eotaxin by GR-MF stimulated with TGF-β (30 ng/mL) and IL-13 (40 ng/mL). The relative value was obtained in relation to GAPDH expression. b Eotaxin levels measured by ELISA into supernatant of GR-MF stimulated with TGF-β (30 ng/mL) and IL-13 (40 ng/mL). c Immunolocalization of eotaxin in GR-MF. Magnification 600X. d Eotaxin levels measured by ELISA into supernatant of GR-MF spheroids stimulated with TGF-β (30 ng/mL) and IL-13 (40 ng/mL) after 4 days in culture. These graphs are representative of three experiments. *P ≤ 0.05 compared with non-stimulated cells