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. 2015 Nov 9;8:577. doi: 10.1186/s13071-015-1197-3

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© Paiva et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Co-culture of GR-MF with non-adherent hematopoietc bone marrow cells obtained from mice infected with S. mansoni in the chronic phase a Clusters of eosinophils observed in monalayer co-culture, after 7 days, stained with May-Grünwald/Giemsa, Hematoxylin/Eosin and Chromotrope 2R. b Eosinophils (arrows) observed in spheroids co-culture, after 4 days, stained with Hematoxilin/Eosin. c Measurement of EPO levels in co-culture treated with neutralizing antibodies for IL-5 and eotaxin. This graph is representative from three experiments. *P ≤ 0.05 compared with non-treated cells