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. Author manuscript; available in PMC: 2015 Nov 10.
Published in final edited form as: Nat Methods. 2015 Sep 29;12(10):901–902. doi: 10.1038/nmeth.3588

Figure 1.

Figure 1

Nuclease contamination of oxygen-scavenging enzymes; purification and analysis of PCD. (a) Analysis of endonuclease activity in different OSS components. The resulting products include the following: SC, supercoiled monomer; NC, nicked circular monomer; ND, nicked dimer. (b) Normalized absorbance at 280 nm (light blue shaded area), percent PCD activity (blue) and percent endonuclease activity (red) of Superose 6 gel filtration fractions. (c) Kinetics of Cy3 photobleaching at different purified PCDa concentrations. (d) The calculated half-life of Cy3 with the purified PCDa OSS in the imaging buffer. The half-life of Cy3 corresponds to a 50% survival probability in c. Error bars denote ±s.e. (e) Extended analysis of the survival probability of Cy3 in the presence of 10 nM purified PCDa OSS (see main text).