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. 2015 Nov 5;56(12):7214–7223. doi: 10.1167/iovs.15-17107

Figure 5.

Figure 5

HSPCs-derived macrophages transfer cystinosin-bearing lysosomes via TNTs to the adjacent disease cells. (a) Tunneling nanotubes generated from the DsRed bone marrow–derived cells were observed in the cornea (sclera margin and ciliary margin) and retina (arrows). (b) Representative confocal pictures of the cornea of Ctns−/− mice transplanted with Ctns−/− DsRed HSPCs as control (upper) or with Ctns−/− DsRed HSPCs stably expressing the fusion protein CTNS-eGFP (lower). Green vesicles are observed within the DsRed-bone marrow–derived cells in the mice treated with CTNS-eGFP-HSPCs but also in the no color host cells (white arrowheads) showing that lysosomes containing CTNS-eGFP could be transferred. Inset shows a TNT containing cystinosin-bearing vesicles (yellow arrowhead). Scale bars: (a) 10 μm, (b) 15 μm.