. 2015 Nov 10;10(11):e0142666. doi: 10.1371/journal.pone.0142666

Table 4. PCR primers used for SNP statistical analysis.

Gene	SNPs	Directions	primers	Size	Tm (°C)
*MSX1*	Rs3821949	Forward	CTCCCCTGACCCCAACTC	388bp	52.8
*MSX1*	Rs3821949	Reverse	CCACCCTCGCTCTGAACT	388bp	52.8
	Rs12532	Forward	CTCCGAAGTCTGATCCCT	215 bp	51.0
	Rs12532	Reverse	CTTTTCTTGCCTGGTGT	215 bp	51.0
*MSX2*	Rs4647952	Forward	TTCGGGAAGAGCCAATCA	376 bp	59.4
*MSX2*	Rs4647952	Reverse	TTCTTGTCGGACATGAGCG	376 bp	59.4
	Rs2048152	Forward	CAGAGGGTGTCTTGGATGCG	249 bp	59.3
	Rs2048152	Reverse	AGATGGAGCGGCGTGGAT	249 bp	59.3
	Rs4242182	Forward	AGAGGGGAGGCCCGAAAG	203 bp	53.9
	Rs4242182	Reverse	GCGAGGAGCTGGGATGTG	203 bp	53.9
	Rs61739543	Forward	ACGCCCTTTACCACATCC	542 bp	55.3
	Rs61739543	Reverse	CCTCCGCCTACAGAACAA	542 bp	55.3
	Rs111542301	Forward	ACCACATCCCAGCTCCTC	346 bp	56.8
	Rs111542301	Reverse	GGTACATGCCATATCCCACT	346 bp	56.8
	Rs3087539	Forward	GCATGTACCACCTGTCCT	268 bp	52.5
	Rs3087539	Reverse	CCATCAGAGCCAATCTTT	268 bp	52.5