Fig 1. Evaluation of the amount of IFN-γ produced in vaccinated mice.

Nine BALB/c female mice per group were vaccinated three times subcutaneously with 100 μg/mouse of P₅₊₄₃₅ long peptide, P₅₊₄₃₅ in combination with CpG, or in combination with both 50 μg/mouse of PADRE and 25 μg/mouse of CpG. Two weeks after the last vaccination, splenocytes from three mice from each group were collected and activated with the long peptide. Immune responses were then determined using an IFN-γ ELISpot assay. The data indicate the mean ± SD. (n = 3). * denotes significant difference from all other groups (P < 0.001).