Table 2. Initial concentrations of the species in the H0 model.

Note that the PIP3 time-course is interpolated from experimental observations and not governed by the equations of the model. Aktp³⁰⁸ is defined to be the sum of Aktp³⁰⁸m and Aktp³⁰⁸c. Initial concentrations of PIP3:PDK1m and Aktp³⁰⁸c were chosen such that the total (membrane plus cytosolic) amount of PDK1 (PDK_total) and Akt (Akt_total) would be 10.

Species	Initial concentration
PIP3	from measured data (linear piecewise spline)
PDK1_total	10
PDK1	9.75
PIP3:PDK1m	[PPDK1_total]—[PDK1]
Akt_total	10
Aktc	9.988
PIP3:Aktm	0.001
Aktp308m	0.001
Aktp308c	[Akt_total]—[Aktc]—[PIP3:Aktm]—[Aktp308m]
Aktp308	[Aktp308m] + [Aktp308c]
Phosphatase	0.24