Fig 3. Compound 2 treatment of L. major-infected BALB/c mice is associated with reduced parasite burden in infected tissues and organs.

Female BALB/c mice (n = 33) were infected with 5 × 10⁶ L. major promastigotes into the right hind footpad. Treatment regimen started on d 21 post infection either using 50% DMSO/PBS (□) (n = 11), 50 mg/kg compound 2 (■) (n = 11) or mice were left untreated (▲) (n = 11). A. At the end of experiment on d 35 all mice (n = 33) were sacrificed and the parasite burden of the infected footpads (iFP) (n = 9) was determined using Limited Dilution Assays (LDA). B. Photographic documentation of the infected footpads of three exemplary mice per group shall reveal the severity of disease progression in non-healing mice and the control of clinical manifestation in compound 2-treated BALB/c mice. C. The parasite burden of the infection site-draining popliteal lymph nodes (pLN) of individual mice (n = 9) or D. spleens of individual mice (n = 6) was determined using LDA. n = number of animals; ** p<0.005; differences in parasite load between 50% DMSO/PBS-treated and compound 2-treated animals did not reach statistical significance for D.