Table 2.

Ca^2 +-dependence of force and the cTnC orientation parameter < P₂ >.

	BR-cTnCC	+ 25 μM blebbistatin	BR-cTnCC (L29Q)	+ 25 μM blebbistatin	BR-cTnCE	+ 25 μM blebbistatin	BR-cTnCE (L29Q)	+ 25 μM blebbistatin
(n = 4)		(n = 4)		(n = 5)		(n = 6)
Force
pCa50	5.64 ± 0.02		5.55 ± 0.03 #		5.67 ± 0.02		5.63 ± 0.02 NS
nH	3.35 ± 0.18		2.88 ± 0.31 NS		3.66 ± 0.29		3.34 ± 0.41 NS
< P2 >
pCa50	5.72 ± 0.01	5.65 ± 0.01 *	5.69 ± 0.01 NS	5.68 ± 0.01 ns	5.69 ± 0.01	5.63 ± 0.01 *	5.67 ± 0.01 NS	5.67 ± 0.02 ns
nH	4.14 ± 0.25	2.66 ± 0.34 *	2.98 ± 0.14 #	2.29 ± 0.10 *	4.20 ± 0.18	3.17 ± 0.27 *	3.37 ± 0.23 #	2.46 ± 0.18 *
at pCa 6.4	0.106 ± 0.004	0.098 ± 0.004 *	0.098 ± 0.001 NS	0.092 ± 0.001 *	0.287 ± 0.008	0.296 ± 0.008 *	0.287 ± 0.008 NS	0.301 ± 0.007 *
at pCa 4.5	0.007 ± 0.009	0.020 ± 0.008 *	0.003 ± 0.003 NS	0.020 ± 0.002 *	0.190 ± 0.005	0.232 ± 0.006 *	0.178 ± 0.008 NS	0.231 ± 0.006 *

Mean ± SEM. pCa₅₀ and n_H are fitted parameters of Hill equation (see Methods and Materials). Comparisons: with and without L29Q mutation (t test, two-tailed; ^NSP > 0.05; ^#P < 0.05); before and after addition of blebbistatin (paired t test, two-tailed; ^nsP > 0.05; * P < 0.05).