Fig. 7. The effect of amino acid changes in predicted TMH4 on (1-3,1-4)-β-glucan structure across species.
The DP3/DP4 ratio of (1-3,1-4)-β-glucan produced in N. benthamiana leaf from wild-type CslF6 proteins with an isoleucine in predicted TMH4 is shown as black bars. When the indicated isoleucine is changed to a leucine, the DP3/DP4 ratio of the (1-3,1-4)-β-glucan is decreased (light gray bars). A further decrease in the DP3/DP4 ratio is observed when a second amino acid substitution is made in the HvCslF6 TMH4 region (HvCslF6S752G,I757L). Shown are averages and SDs of replicates from at least two transformation experiments. The amount of (1-3,1-4)-β-glucan produced for the respective constructs was 1.8, 2.2, 0.4, 0.6, 1.8, 2.4, and 1.8% of the dry weight of the freeze-dried leaf tissue.