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The wild-type and the mutant Runx2 protein expression. C3H10T1/2 cells were transiently transfected with either the c-myc-tagged wild-type Runx2 construct or the c-myc-tagged mutant Runx2 construct for 24 h. As negative controls, cells (C3H10T1/2 and UMR 106-01) were transiently transfected with empty vector. Whole cell lysates were prepared and Western blot analysis was carried out using the antibodies as indicated. β-Actin has been used as loading control. IB, immunoblot.
