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. 2015 Nov 10;10(11):e0141231. doi: 10.1371/journal.pone.0141231

Fig 6. Macrophage depletion showed no effect on interstitial fibrosis and myofibroblast accumulation in Col4a3KO mice.

Fig 6

A (Sirius red) and C (α-SMA) show increased deposition of Sirius red stained fibrotic tissue and α-SMA cells in the interstitium of KO+PBSL; B (Sirius red) and D (α-SMA) show an increased staining of the interstitium in KO+CL that was comparable to KO+PBSL, (circle: sclerotic glomeruli; star: normal-appearing glomeruli). Glomerular crescents (arrows) were also observed occasionally in glomeruli. (E) Morphometric analysis of α-SMA stained myofibroblasts confirmed no discernable effect of CL treatment on myofibroblasts deposition. (F-H) Expression of profibrotic genes (F), genes for ECM remodeling (G) and inflammation (H) is comparable in KO+PBSL and KO+CL mice. Welch corrected ANOVA followed by posthoc pairwise t-test with a Bonferroni correction was used to evaluate differences between groups. (E-H) n = 5 mice per group. Scale bar 100 um. CL: clodronate liposomes; PBSL: PBS liposomes.