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. 2015 Oct 19;112(44):13699–13704. doi: 10.1073/pnas.1508347112

Fig. 4.

Fig. 4.

Single-cell amplification studies confirmed sperm miR targeting of stored maternal mRNA. (A) Expression of many more maternal mRNA candidate genes in zygotes was reduced by multi-miR injection than by the single-miR injection. P < 0.005. (B–I) Expression of 8 of the 75 candidate genes—Sirt1, Ube3a, Srsf2, IL6st, Ncl, Aars, Agfg1, and Ralbp1—was significantly reduced by the multi-miR injection, with expression levels measuring log2 fold change > |1| (dotted lines) relative to PBS controls. Expression of these genes was unaffected in single-miR injection controls. Data are shown relative to PBS-injected zygotes and presented as mean ± SEM, n = 10–13 zygotes per group. *P < 0.05 multi-miR vs. PBS, P < 0.05 multi-miR vs. single miR.