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. 2015 Oct 19;112(44):E5936–E5943. doi: 10.1073/pnas.1516319112

Fig. 3.

Fig. 3.

Erk signaling is required for ESC differentiation. (A and B) iErk1; Erk KO ESCs were cultured with or without Dox for 48 h. The cells were then used for EB differentiation, again with or without Dox. (A) Images of day 4 EBs with or without Dox. The relative diameters of day 4 EBs with or without Dox (n > 10) are plotted. (B) RNA was isolated from day 4 EBs and analyzed by quantitative RT-PCR. TE, trophectoderm. (C) iErk1; Erk KO ESCs were cultured with or without Dox for 48 h (D0, day 0). These cells, as well as KH2 ESCs, were cultured in N2B27 with or without Dox to induce neural differentiation for 4 d. The expression of pluripotency genes and neural genes in the resulting cells was analyzed by quantitative RT-PCR. *P < 0.05; **P < 0.01; ***P < 0.001. Error bars are SDs.