Fig. S1.

Knockout of Erk1 and Erk2 by CRISPR/Cas and TALENs. (A) Schematic illustration of CRISPR/Cas and TALEN design for Erk1. (B) Schematic illustration of CRISPR/Cas and TALEN design for Erk2. CRISPR/Cas and TALEN targeting sites are labeled with red triangles and black arrows, respectively. Recognition sequences for CRISPR/Cas and TALENs are underlined. (C) Experimental outline for the construction of iErk1; Erk KO ESCs. (D) Sequencing results of two clones of iErk1; Erk KO ESCs demonstrate the disruption of the Erk2 alleles. Blue triangles mark the deletion sites. PAM, protospacer adjacent motif.